利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN108004262A 一种根癌农杆菌介导的平菇菌丝遗传转化方法 无效 - 驳回

一种根癌农杆菌介导的平菇菌丝遗传转化方法
摘要：
本发明提供了一种利用根癌农杆菌介导的平菇菌丝遗传转化方法，属于基因的遗传转化技术领域，包括以下步骤：1)质粒载体改造；2)改造后的质粒载体转化农杆菌，获得阳性菌；3)阳性菌侵染平菇菌丝，共培养得新长出的菌丝；4)将所述新长出的菌丝接种到含有潮霉素和头孢霉素的PDA培养基上选择培养5～7d，分离得到有抗生素抗性的平菇转化菌株；5)对所述平菇转化菌株进行转基因鉴定。本发明方法适用于平菇基因功能的验证及遗传改良育种等研究，具有操作简单、稳定性强、转化率高、成本低廉等优点。
摘要（英）：

The invention provides an agrobacterium tumefaciens-mediated oyster mushroom mycelium genetic transformation method and belongs to the technical field of genetic transformation of genes. The method comprises the following steps: 1) plasmid vector modification; 2) transforming agrobacterium by the modified plasmid vector so as to obtain positive bacteria; 3) infecting oyster mushroom mycelia by thepositive bacteria, and co-culturing to obtain new grown mycelia; 4) inoculating the new grown mycelia into a PDA culture medium containing hygromycin and cephalosporin to perform selective culture for 5-7 days, and separating to obtain oyster mushroom transformation strains; 5) performing transgenic confirmation on the oyster mushroom transformation strains. The method disclosed by the inventionis applicable to verification of oyster mushroom gene functions, genetic improvement breeding and other researches, and has the advantages of being simple in operation, high in stability, high in transformation rate, low in cost and the like.

0/0