The invention discloses a quadruple fluorescent PCR primer set for detecting four pathogenic bacteria in drinking water. The quadruple fluorescent PCR primer set comprises primers for coli groups, streptococcus faecalis, pseudomonas aeruginosa and clostridium perfringens, and is specifically shown as Seq. ID No. 1 to Seq. ID No. 8. The invention belongs to the technical field of gene engineering detection, mutual interference cannot be caused for each primer and each probe, amplification can be carried out on specific objective sequences only, a fluorescence signal is stimulated, non-objectivesequences are free of amplification or fluorescence signal, accurate detection for the four pathogenic bacteria in drinking water can be realized, and the quadruple fluorescent PCR primer set has theadvantages of high specificity, small standard error, short detection time, reagent cost saving and the like and is applicable for detecting the indexes of the pathogenic bacteria in the standard ofdrinking water.