The invention discloses a method for leaching copper in a waste printed circuit board by means of acidithiobacillus ferrooxidans. According to the method, the acidithiobacillus ferrooxidans is promoted to leach the copper in the waste printed circuit board through two-stage dissolved oxygen control. The method for leaching the copper in the waste printed circuit board by means of the acidithiobacillus ferrooxidans comprises the steps that firstly, the acidithiobacillus ferrooxidans obtained after copper resistance domestication is completed is cultured under the low dissolved oxygen condition till the acidithiobacillus ferrooxidans growth mass approaches the maximum value; secondly, waste printed circuit board powder is added to an acidithiobacillus ferrooxidans culture system, the content of dissolved oxygen in the acidithiobacillus ferrooxidans culture system is increased after the waste printed circuit board powder is added, and the copper in the waste printed circuit board is leached by means of the acidithiobacillus ferrooxidans under the high dissolved oxygen condition. By the adoption of the method, the acidithiobacillus ferrooxidans concentration and the copper leaching rate are both maximized, and the overall leaching time is greatly shortened.