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CN108020664A 检测II型草鱼呼肠孤病毒疫苗中病毒含量的试剂盒及方法 无效 - 驳回

检测II型草鱼呼肠孤病毒疫苗中病毒含量的试剂盒及方法
摘要：
本发明涉及一种检测II型草鱼呼肠孤病毒疫苗中病毒含量的试剂盒及方法，进一步地涉及活疫苗或灭活疫苗(病毒灭活前)的病毒含量的测定，所述试剂盒包括用于检测基因II型GCRV VP41的血清抗体、FITC标记的羊抗兔IgG、阳性对照样品、固定液和PBS洗液。检测方法为将活疫苗或灭活疫苗(病毒灭活前)进行梯度稀释后接种于PSF细胞，连续培养完成后再用本发明所述的试剂盒进行免疫荧光检测，将检测结果进行计算得出相应的病毒含量。本发明的试剂盒及方法灵敏度高、特异性强，且能定量检测活病毒。
摘要（英）：

The invention discloses a kit and a method for detecting virus content in reovirus vaccine of II-type grass carps, further relates to the measurement of virus content of live vaccine or inactivated vaccine (before virus is inactivated), the kit comprises serum antibody for detecting gene II-type GCRV VP41, FITC labeled goat anti-rabbit IgG, positive control samples, stationary liquid and PBS lotion. According to the detection method, after gradient dilution of the live vaccine or the inactivated vaccine (before virus is inactivated) is conducted, then the live vaccine or the inactivated vaccine (before virus is inactivated) is inoculated into PSF cells, after continuous culture is completed, immune fluorescence detection is conducted with the kit, and the detection results are calculated and the corresponding virus content is obtained. According to the kit and method, the sensitivity is high, the specificity is high, and live virus can be detected quantitatively.

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