The invention relates to the field of food processing, in particular to a selenium-rich steamed bread and a preparation method of the selenium-rich steamed bread. A total selenium content of the selenium-rich steamed bread is 0.01-0.1mg/kg, and a content of organic selenium accounts for 80% or above of the total selenium content. The content of organic selenium is high, so that the food safety isensured. The preparation method of the selenium-rich steamed bread comprises the steps of producing selenium-rich wheatmeal with medium mature selenium-rich wheat as a raw material, preparing preferment by direct fermentation of a compound selenium-rich flora, or preparing selenium-rich ferment by fermentation of fruits and vegetables with compound selenium-rich bacterium powder to prepare the preferment, and fermenting the selenium-rich wheatmeal with the preferment to prepare the selenium-rich steamed bread. The selenium-rich dough steamed bread produced by the method is good in taste and flavor and high in nutritive value and can further safely and efficiently supplement selenium required by a human body.