The invention provides a layered HZSM-5 catalyst. The layered HZSM-5 catalyst is prepared through the following treatment processes: preparing a silicon source and a template agent into a mixed solution, wherein the silicon source is introduced in a gaseous state, then carrying out standing and aging, carrying out a closed reaction at a high temperature and high pressure, soaking an obtained material into an NH4Cl solution, carrying out ion exchange, and carrying out washing, drying, calcination, etc. The layered HZSM-5 catalyst provided by the invention has a silicon-to-aluminum ratio of (25-40): 1, a specific surface area of 350 to 450 m2/g and a pore volume of 0.25 to 0.40 cm3/g, wherein a mesopore volume accounts for 50 to 80% of the pore volume. In the preparation process of the layered HZSM-5 catalyst provided by the invention, a molecular sieve has easily-controllable silicon-to-aluminum ratio; and the catalyst has high silicon-to-aluminum ratio, a plurality of mesopores and good pore size distribution, and is used in a reaction for preparation of aromatic hydrocarbon through biomass catalysis, and greatly improves the yield of the aromatic hydrocarbon.