The invention belongs to the field of enzyme engineering and particularly relates to a DNA polymerase immobilization method and an application thereof. The immobilization method comprises steps as follows: a modified chitosan water solution is prepared firstly, an adsorption reaction solution of DNA polymerase is prepared, a crosslinking reaction solution of the DNA polymerase is prepared, the crosslinking reaction solution and wood pulp sponge are mixed, a carrageenin water solution is added, the mixture is centrifuged and washed and finally freeze-dried, and the immobilized DNA polymerase isobtained, and the prepared immobilized DNA polymerase has higher activity and better thermal stability; the prepared immobilized DNA polymerase has the highest activity and the best thermal stabilityby selecting a specific protein flocculant, specific adsorption reaction conditions, specific crosslinking reaction conditions, a specific crosslinking agent, a specific buffer solution, specific carriers, a specific PCR accelerator and specific freeze-drying conditions.