The invention provides a regeneration method of an aluminum oxide loaded platinum-tin dehydrogenation catalyst, and belongs to the technical field of catalyst regeneration. The regeneration method comprises burned carbon chlorination and reduction, wherein the burned carbon chlorination is to perform burned carbon chlorination by making a deactivated catalyst contact with regeneration gas; the reduction is to perform catalyst reduction by making the burned carbon contact with the chlorinated catalyst and the reduction gas, and purge a catalyst bed layer with nitrogen gas after finishing of thereduction in order to lower the hydrogen gas volume in the system at 0.5 percent or less. By adopting the regeneration method provided by the invention, carbon burning and chlorination are realized simultaneously. The regeneration method has the advantages that firstly, in the presence of a chlorinating agent, the phenomenon that a part of metal atoms are sintered excessively during separate burning of the carbon can be avoided, the activity of the catalyst after regeneration is increased, and the service life of the catalyst is prolonged; secondly, carbon burning and chlorination are performed simultaneously, so that the treatment time can be shortened, and energy waste is reduced in comparison with separate treatment.