The invention relates to a method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress. The method is characterized by comprising the steps: controlling a potassium ion concentration to be 100-2000 mg/kg in a process of culturing mature momordica grosvenori suspension cells by a primary momordica grosvenori suspension cell system, wherein a potassium-sodium ion concentration ratio is (5-20):1. According to the method disclosed by the invention, the growth amount and the specific growth rate of momordica grosvenori and the yield and synthesis rate of the mogroside V are improved, and momordica grosvenori suspension cell growth and large-scale market production of the mogroside V are facilitated; meanwhile, the production and culture cycle of cells is effectively shortened, the biomass of the momordica grosvenori suspension cells and the production process of the mogroside V are regulated in a manual manner, and quality control and management of the product can be facilitated.