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发明公开
CN108315474A 水稻孕穗期耐冷基因qCT3.12FAZ的分子标记及应用 有权 转让 许可

水稻孕穗期耐冷基因qCT3.12FAZ的分子标记及应用
摘要：
本发明公开了水稻孕穗期耐冷基因qCT3.12FAZ的分子标记，为采用引物对RM227以带有籼稻品种丰矮占血缘的育种材料基因组DNA为模版扩增出来的95bp核苷酸序列；引物对RM227的正向引物序列如SEQ ID No.1所示，引物对RM227的反向引物序列如SEQ ID No.2所示。本发明的分子标记可用于孕穗期育种群体的基因型选择，有效地鉴别带有该基因的耐冷个体，便于及时的杂交转育，加快育种进程。
摘要（英）：

The invention discloses a molecular marker for cold-resistant gene qCT3.12 at a booting stage in rice. The molecular marker is a 95bp nucleotide sequence which is amplified by using a breeding material gene group DNA with consanguinity of indica rice varieties as a template through a primer pair RM227; a forward primer sequence of the primer pair RM227 is shown in SEQ ID No. 1, and a reverseprimer sequence of the primer pair RM227 is shown in SEQ ID No. 2. The molecular marker disclosed by the invention can be applied to selection of gene types of breeding colonies in the booting stage in rice, cold-resistant individuals with the gene are effectively identified, hybridization and breeding transferring are carried out in time, and breeding procedures are quickened.

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