The invention discloses a preparation method of a Ta-based high-temperature protection coating with a self-healing function. The preparation method of the Ta-based high-temperature protection coatingwith the self-healing function comprises the steps of adopting a 99.99-percent high-pure Ta circular block material as a magnetron sputtering target material, adopting a direct-current magnetron sputtering method, pre-sputtering on a clean stainless steel substrate, preparing a layer of completely covered Ta thin film, and obtaining a Ta middle layer with an alpha phase; and selecting the 99.99-percent high-pure Ta circular block material and a high-pure Ag target material as sputtering raw materials, adopting two direct-current magnetron sputtering power supplies for independently regulatingthe two target materials, and preparing a Ta-Ag self-healing high-temperature protection layer on the Ta middle layer with the alpha phase. According to the preparation method of the Ta-based high-temperature protection coating with the self-healing function provided by the invention, the structure regulation of the self-healing layer can be realized; in a Ta-Ag composite thin film structure, Ag is dissolved into a crystal lattice of the metal Ta so as to form a super-solid solution; during the generation of high-temperature cracks, vacancies and microcracks can be filled to the vacancies through the formation of the precipitated-phase Ag so as to achieve healing; and generated tantalum oxide can form a protection layer on the surface of the thin film, so that the further oxidization is prevented.