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发明公开
CN108387725A 一种脊髓灰质炎病毒Ⅰ型、Ⅱ型、Ⅲ型D抗原预包被联合检测方法及其检测试剂盒和应用 无效 - 撤回

一种脊髓灰质炎病毒Ⅰ型、Ⅱ型、Ⅲ型D抗原预包被联合检测方法及其检测试剂盒和应用
摘要：
本发明提供一种预包被的可进行批量生产的脊髓灰质炎病毒（Polio virus）Ⅰ型、Ⅱ型、Ⅲ型D抗原检测方法，采用牛（兔、羊）抗Ⅰ型、Ⅱ型、Ⅲ型脊髓灰质炎病毒D抗原纯化抗体包被微孔板，并通过保护剂对包被板和包被抗体进行保护后制备成预包被板，同时配套辣根过氧化物酶标记的牛（兔、羊）抗Ⅰ型、Ⅱ型、Ⅲ型脊髓灰质炎病毒酶标抗体和其他试剂。该方法（试剂）可以对脊髓灰质炎病毒Ⅰ型、Ⅱ型、Ⅲ型D抗原进行特异性的定性和定量检测，对脊髓灰质炎病毒Ⅰ型、Ⅱ型、Ⅲ型C抗原和其他肠道病毒无交叉，是一种特异性高、灵敏高，使用方便、用途广泛的Ⅰ型、Ⅱ型、Ⅲ型脊髓灰质炎病毒D抗原联合检测法，在IPV疫苗生产检定中有较高的应用价值。
摘要（英）：

The invention provides a pre-coating Polio virus I, II and III D antigen detection method for batch production. Bovine (rabbit and sheep) anti-I, II and III Polio virus D antigens are employed to purify an antibody-coated microporous plate, also a protective agent is employed for protection of a coated plate and coating antibodies so as to prepare a pre-coated plate, and the pre-coated plate is matched with horseradish peroxidase labeled bovine (rabbit and sheep) anti-I, II and III Polio virus enzyme-labeled antibodies and other reagents at the same time. The method (reagent) can achieve specific qualitative and quantitative detection of Polio virus I, II and III D antigens, has no cross with Polio virus I, II and III C antigens and other enteroviruses, and is a type I, II and III Polio virus D antigen combined detection method with the advantages of high specificity, high sensitivity, convenient use and extensive use, and has high application value in production and verification of IPV vaccines.

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