The present invention relates to a hanging drop protection cover automatic separation device. The device comprises a clamping body, a hanging device, a protective cover connection rod and the like. The left and right sides of the clamping body are provided with support lugs, and the upper and lower surfaces of the clamping body are provided with a middle through hole and a side through hole; the front side of the clamping body is provided with a fastening hole and a regulation hole; the hanging device comprises a metal rod and a quartz frame; the tail end of the metal rod is fixedly connectedwith the top plate of the quartz frame; the metal rod penetrates the middle through hole, and is fixed through a fastening screw in the fastening hole; the protective cover connection rod penetrates the side through hole and is regulated through a regulation screw in the regulation hole; the middle section of the protective cover connection rod is provided with a supporting rod being perpendicularto the protective cover connection rod, and a positioning screw is fixed on the supporting rod; an inlet panel is fixed at the upper portion of a high-temperature cavity and is located below the positioning screw; the bottom end of the protective cover connection rod is fixedly connected with the top end of a quartz glass cover; and a through hole configured to put a thermocouple is formed at themetal rod, the middle portions of the quartz frame and the quartz glass cover. The hanging drop protection cover automatic separation device allow the drop to be closer to the state of an initial position at a target position when evaporation starts.