The invention belongs to the field of polypeptide compound synthesis, and discloses a synthesis method of wide activity range cyclo-heptapeptides Reniochalistatin A-D. The structure formula of the Reniochalistatin A-D is disclosed in the invention. A polypeptide solid phase synthetic method is adopted, fiuorenylmethoxy carbony-L-proline (Fmoc-Pro-OH) is taken as a raw material, and is used for blocking reaction sites on 2-chloride resin, Fmoc deprotection is repeated, condensation reaction with an amino acid with Fmoc protection is carried out until a linear heptapeptide-resin composite is obtained, cutting of resin is carried out, and cyclization reaction is carried out using a cyclization agent until finished products cyclo-heptapeptides Reniochalistatin A-D are obtained. Operation of the synthesis method is simple; yield is high; the raw materials are cheap; and the condensation agent is a common reagent.