The invention discloses a one needle and one thread menisci stitching instrument, which belongs to the field of medical instruments and comprises a sewing needle and a guiding needle, wherein the sewing needle is provided with an axial through hole which penetrates through the needle and the guide needle, the bottom surface of the groove penetrates through the side wall of the axial through hole,the upper end of the groove penetrates through the oblique incision, and the lower end of the guide needle is provided with a tapered sharp part. The radial dimension of the sharp part is smaller thanradial dimension of the axial through hole, and the oblique incision is in smooth transition with the inner wall of the axial through hole and the outer wall of the sewing needle. The stitching instrument enables the operator to quickly and accurately determine the needle insertion angle, the needle inserting point, the needle inserting path and the needle out point under the condition that the line of sight is blocked.