The invention belongs to the field of sealing vibration isolation, and particularly discloses a double-layer sealing structure capable of being inflated and deflated and a gas spring. The double-layersealing structure comprises a gas nozzle body, an inner sealing unit and an outer sealing unit; the inner sealing unit comprises an elastic gas nozzle with a hole and a stopping block with a hole, the elastic gas nozzle is arranged on the side, close to compressed gas, of the gas nozzle body, the stopping block with the hole is arranged in the gas nozzle body, and a first sealing sheet with a hole is arranged between the stopping block with the hole and the elastic gas nozzle; the outer sealing unit comprises a threaded fastening part, the threaded fastening part is arranged on the side, faraway from the compressed gas, of the gas nozzle body, and a second sealing sheet with a hole is arranged between the threaded fastening part and the stopping block with the hole in the inner sealing unit; and the gas spring comprises the double-layer sealing structure and a flexible gas bag. The double-layer sealing structure has the advantages that the gas tightness is good, inflating and deflating can be achieved, and no gas leakage phenomenon is generated.