The invention relates to the field of environmental protection, and discloses an antibiotic degradation mixed bacterial agent and application thereof. The mixed bacterial agent is prepared from Arthrobacter sp. and Alcaligenes sp.. The Arthrobacter sp. is tetracycline antibiotic degradation arthrobacterium, and can be used for simultaneously degrading oxytetracycline, tetracycline and aureomycin;the Alcaligenes sp. is sulfanilamide antibiotic degradation bacterium, can be used for degrading sulfadimethoxine, and can tolerate sulfadimidine, sulfadiazine and sulfamonomethoxine. The antibiotic degradation strains and bacterial agents can be used for degradation removal of residue antibiotics in waste such as livestock and poultry excrement; the release of the residue antibiotic in the wasteto the environment can be reduced; meanwhile, the bacterial agent can be applied to the degradation removal of the antibiotic contaminants in the antibiotic polluted soil; when the bacterial agent isused for environmental restoration, good application prospects and environmental benefits are realized.