The invention discloses a self-healing concrete compound-enzyme microcapsule material and a preparing method thereof. The self-healing concrete compound-enzyme microcapsule material is prepared from,by weight, 0.5%-1% of urease, 0.5%-1% of formate dehydrogenase, 0.5%-1% of oxidation-type nicotinamide adenine dinucleotide, 0.05%-0.3% of polyvinyl alcohol with the alcoholysis degree larger than 99.5%, 18%-24% of urea, 39%-52% of calcium formate, 5%-10% of calciferous retarder and 10%-30% of silica fume, wherein the urease, the formate dehydrogenase, the oxidation-type nicotinamide adenine dinucleotide and the polyvinyl alcohol are processed into microcapsules with the spray drying technology and then mixed with other components. According to concrete with the self-healing concrete compound-enzyme microcapsule material, fissures of the concrete can be self-healed in the presence of water, and therefore the durability of the concrete is improved.