The invention discloses a method for lowering the detection limit of lateral flow chromatographic test paper, and belongs to the technical field of quick detection. The method includes sequentially arranging sample pads, chromatographic films and absorbent pads (without conjugation pads) in a linearly arraying manner; loading sample solution on the sample pads to preliminarily enrich target detection objects on detection zones (T zones) of the chromatographic films; inserting the conjugation pads between the sample pads and the chromatographic films; continuing to load sample solution or buffer solution on the sample pads. Compared with other methods for lowering the detection limit of existing lateral flow chromatographic test paper on the basis of sample enrichment and concentration, themethod has the obvious advantages that the detection limit can be lowered to a great extent; other amplification reagents, materials and equipment can be omitted, the detection cost does not need tobe increased, the method is simple, convenient and speedy and is high in efficiency, and the like.