The invention discloses a route selection method based on MAC layer contention access and mobility in Internet of Vehicles. The implementation method of the strategy is as follows: first, a transmitting end vehicle collects speed and direction information of a neighboring vehicle in a hop communication range and calculates corresponding link duration and distance; secondly, estimation is carried out on the number of back-off times of the MAC layer so as to obtain the average number of back-off times of the MAC layer of the neighboring vehicle; on this basis, the weight of each vehicle is obtained in a weighting manner, and routing selection is carried out based thereon. The route selection method based on MAC layer contention access and mobility in Internet of Vehicles in the present invention optimizes the routing selection strategy by combining mobility characteristics of vehicle speed and direction, and a back-off mechanism for the MAC layer contention access of the vehicle. The simulation results show that the proposed strategy has better system performance, compared with the traditional GPSR scheme.