The invention discloses a perovskite light-emitting diode with a QD-3D-QD light-emitting layer. The QD-3D-QD light-emitting layer is of a quantum dot perovskite-three-dimensional perovskite-quantum dot perovskite structure, namely a QD-3D-QD structure for short, wherein the light-emitting layer is prepared through the following processes of firstly carrying out spin coating of a quantum dot perovskite layer; carrying out spin coating of a layer of three-dimensional organic-inorganic hybrid perovskite on the quantum dot perovskite layer; and carrying out spin coating of a layer of quantum dot perovskite on the two layers. The perovskite light-emitting diode is suitable for two currently used basic device structures of the perovskite light-emitting diode, wherein one basic device structure sequentially comprises a substrate, an anode, a hole transport layer, a perovskite light-emitting layer, an electron transport layer and a cathode and the other basic device structure sequentially comprises the substrate, the cathode, the electron transport layer, the perovskite light-emitting layer, the hole transport layer and the anode.