The invention discloses a thrips-killing plant essential oil composition, a preparation and an application thereof. The thrips-killing plant essential oil composition contains wintergreen oil and origanum oil, and the weight ratio of wintergreen oil to origanum oil is 1:6 to 6:1. The composition disclosed by the invention can prevent and control thrips, has an obvious synergistic effect, is not easy to generate drug resistance, can improve the capability of the immune system of a crop, can remarkably improve the vitality of the crop, and is a pollution-free plant-derived thrips prevention andcontrol agent which is safe to human beings and animals and has no pollution to the environment.