The invention discloses a control system and method for culture parameters of a bioreactor. The system comprises a main control unit, sub-control units, a tray and a cell culture bag, wherein the maincontrol unit is used for receiving parameter information set by a user and controlling each sub-control unit to perform corresponding operations according to the parameter information; the sub-control units comprise a temperature control unit, a liquid control unit, a gas control unit and a shaking control unit; the temperature control unit, the liquid control unit, the gas control unit and the shaking control unit are controlled by one main control unit. The bioreactor is different from the existing disposable bioreactor in system architecture and structural form, and each sub-control unit belongs to closed loop control, so that the whole system has high control precision, good control stability and quick system response.