The invention discloses a method and a device for simultaneously automatically and quickly measuring hyperoxide enzymatic activity concentration and ascorbic acid contents in a biological sample, belongs to the technical field of biochemical analysis and quantification, and is used for physiological and biochemical research and enzymatic method application. The method comprises the following steps: using two carrying currents to respectively push enzyme reagent and an ascorbic acid sample in a quantitative loop to enter a reaction coil pipe; carrying out enzyme catalytic color development reaction and ascorbic acid fading reaction in sequence; and finally, through a circulation type luminosity detector, quantifying the ascorbic acid through the absorbancy reduction value of a colorful product, meanwhile, after the quantitative loop is full of the enzyme sample, rotating a valve to the original position, pushing the enzyme sample by the carrying current to a reaction coil to carry out the enzyme catalytic color development reaction, and utilizing the increment of the absorbancy of the product to quantify the activity of enzyme. A measurement process and result can be processed by acomputer so as to realize that specimen collection, quantification and measurement are automatically and synchronously carried out.