The invention discloses a method for fast domesticating adherent cells into a full suspension cell line. Low-generation-passage health adherent cells are directly inoculated into a rotating bottle ofa serum-free and micro-carrier-free culture medium to be domesticated and cultured; when the cells adapt to the rotating speed of a rotating bottle machine being greater than 6 rpm, the materials aretransferred into a shaking bottle; the cell initial density is regulated to 5*10<5> cells/mL by a fresh culture medium; the shaking bottle rotating speed is regulated to 10 to 40 rpm; if the cell increase after 4 to 5 days cannot reach 1*10<6> cells/mL, the cells are subjected to centrifugation and precipitation; then, the fresh serum-free and micro-carrier-free culture medium is used for regulating the cell density to 5*10<5> cells/mL; then, continuous domestication and culture is performed; the domesticating step is repeated until the cell density is increased at 10 times or more than 10 times after 4 to 6 days; after ten generations or more of shaking bottle generation passage domesticating culture, the full suspension cell line can be obtained. The method is simple, convenient and fast; the repeated performance is high; the successful rate is high; the full suspension cell line is stable; the infection performance on viruses is unchanged.