The invention relates to the field of catalytic oxygen reduction materials applied to fuel cells and discloses a nitrogen-doped pattern type carbon nanomaterial with a penetrating mesopore. The microstructure of the material is of a nanoflower, and the penetrating mesopore is formed in the nanoflower. The invention further discloses a preparation method of the carbon nanomaterial with the penetrating mesopore. The preparation method comprises the steps of carrying out polymerization reaction by taking an Ni<2+>-Mn<3+> derived lamellar double-layer metal hydroxide nanoflower as a self-sacrificing template, a spherical micelle formed by PS-b-PEO through assembling in a solution as a mesoporous soft template as well as m-phenylenediamine as a carbon precursor so as to obtain a pattern type carbon nanomaterial, cleaning, drying, and carrying out atmosphere calcining. The nitrogen-doped pattern type carbon nanomaterial with the penetrating mesopore is of a three-dimensional structure, has ahigh material specific surface area and high oxygen reduction catalytic performance, the mass transfer efficiency can be improved, the mesopore is unlikely to be blocked, the material performance canbe maintained, and the carbon nanomaterial is easily prepared by virtue of the preparation method.