The invention relates to a method for preventing and controlling cotton verticillium wilt. The method comprises the following step: dripping a first water-soluble compound fertilizer, a second water-soluble compound fertilizer, a third water-soluble compound fertilizer and a fourth water-soluble compound fertilizer into cotton root soil together with water at the seeding stage, bud stage, flowering stage and boll stage of cotton, wherein the first water-soluble compound fertilizer, the second water-soluble compound fertilizer and the third water-soluble compound fertilizer all contain a bio-organic micro fertilizer; the micro fertilizer is prepared from a water-soluble organic matter, fulvic acid, medium trace elements and a microbial preparation. The water-soluble compound fertilizers aredripped into the cotton root soil by an integral water-fertilizer irrigating technology, and various components play a synergistic effect, so that significant growth of cotton is promoted, and the pathogenic bacteria resistance of cotton is improved. Meanwhile, introduced beneficial microbial flora can breed rapidly in order to block and isolate the contact between the pathogenic bacteria and cotton root, so that the infection incidence is lowered, and the effect of preventing and controlling cotton verticillium wilt is achieved.