The invention relates to a method for preparing a soil remediation fertilizer based on lumbrukinase-attapulgite. The method comprises the following steps: weighing attapulgite raw ore, drying and grinding into powder of 120-200 meshes, and adding into distilled water for soaking; weighing a sodium hexametaphosphate dispersing agent to be added into an aqueous solution of the attapulgite powder, wherein the mass of the sodium hexametaphosphate is 1-4% of the mass of the attapulgite; stirring and filtering to obtain a wet filter cake; adding the wet filter cake into a lumbrukinase extracting solution, stirring the mixed solution, and performing vacuum freeze drying; and finally, grinding and granulating, thereby obtaining the lumbrukinase-attapulgite soil remediation fertilizer. According tothe combination of the attapulgite and lumbrukinase, the utilization efficiency of the lumbrukinase soil remediation fertilizer can be greatly improved, inorganic or organic pollution of the soil isremediated, damage of the chemical fertilizer to the soil is alleviated, the soil environment for crop growth is improved, fertilizer and water retaining can be realized, beneficial trace elements areprovided for growth and development of crops, and the fertilizer is a green, safe and environmental-friendly soil remediation fertilizer.