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发明公开
CN108949687A 诱导多能干细胞向运动神经细胞分化的无血清培养体系 无效 - 撤回

诱导多能干细胞向运动神经细胞分化的无血清培养体系
摘要：
本发明属于干细胞生物学和细胞培养技术领域，提出了促进诱导多能干细胞向运动神经细胞分化的无血清培养体系，具体包括无血清分化基本培养基及促进运动神经细胞分化的添加物。其中，无血清基本培养基必要成分包括：DMEM/F12培养基，人血清白蛋白，L‑谷氨酰胺，D‑葡萄糖，维生素C，N2/B27细胞培养添加剂，非必需氨基酸。促进运动神经细胞分化的添加物具体包括：SB431542，皮啡肽，成纤维生长因子‑2，维甲酸，BDNF，GDNF，CTNF，DAPT，Smoothened激动剂，阿糖胞苷。用此培养基可以帮助提高细胞混合体系中的运动神经细胞分化，减少向其他类型细胞的分化，从而最终得到高纯度的运动神经细胞。
摘要（英）：

The invention belongs to the technical fields of stem cell biology and cell culturing, and provides a serum-free culturing system inducing differentiation of pluripotent stem cells into motor neuron cells. The serum-free culturing system specially comprises a serum-free differentiation basic culture medium and an additive for promoting the differentiation of the motor neuron cells, wherein necessary components of the serum-free differentiation basic culture medium comprise a DMEM/F12 culture medium, a human serum albumin, L-glutamine, D-glucose, vitamin C, N2/B27 cell culture additive and unnecessary amino acid. The additive for promoting the differentiation of the motor neuron cells specially comprises SB431542, dermomorphin, fibroblast growth factors-2, retinoic acid, BDNF, GDNF, CTNF, DAPT, Smoothened agonist, and cytarabine. The culture medium is adopted to help improve the differentiation of the motor neuron cells in a cell mixing system, the differentiation of other types of cells is reduced, and finally, the motor neuron cells with high purity can be obtained.

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