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发明公开
CN109022446A 水稻转基因细胞制备方法 无效 - 驳回

水稻转基因细胞制备方法
摘要：
本申请公开了一种水稻转基因细胞制备方法，筛选培养基包括：NB；2，4‑D 1.8‑2.0mg/mL；6‑BA 0.1‑0.2mg/mL；Hyg 20‑25mg/L，Timentin 200‑400mg/L，琼脂粉10‑15g/L。分化培养基包括：NB；Pro 0.3‑0.5g/L；CH 0.2‑0.3g/L；6‑BA 1.8‑2.0mg/mL；KT 0.8‑1.0mg/mL；NAA 0.3‑0.5mg/mL，IAA 0.4‑0.5mg/mL；Hyg 20‑25mg/L；Timentin 200‑400mg/L；蔗糖25‑30g/L；琼脂粉10‑15g/L。本发明方法可以在植物转基因初期可以观察到转基因是否成功的一种快捷的报告基因检测方法，为后续的高效率的阳性植株筛选提供了依据。
摘要（英）：

The present application discloses a method for preparing rice transgenic cells. A screening medium comprises NB, 1.8-2.0mg/mL of 2, 4-D, 0.1-0.2mg/mL of 6-BA, 20-25mg/L of Hyg, 200-400mg/L of Timentinand 10-15g/L of agar powder. A differentiation medium comprises NB, 0.3-0.5g/L of Pro, 0.2-0.3g/L of CH, 1.8-2.0mg/mL of 6-BA, 0.8-1.0mg/mL of KT, 0.3-0.5mg/mL of NAA, 0.4-0.5mg/mL of IAA, 20-25mg/Lof Hyg, 200-400mg/L of Timentin, 25-30g/L of sucrose and 10-15g/L of agar powder. The method is a fast reporter gene detection method for observing whether the transgenosis is successful in the earlystage of plant transgenosis and provides the basis for subsequent high-efficiency screening of positive plants.

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