The invention discloses a reactor for culturing intestinal flora and a method for culturing intestinal flora by utilizing the reactor, and belongs to the technical field of biological fermentation. According to the reactor for culturing the intestinal flora and the method for culturing the intestinal flora by utilizing the reactor, by changing the diameter of a pipeline, fermentation liquor residence times in different intestinal canals are mainly adjusted and simulated so as to preferably simulate influences caused by intestinal canal residence times; in addition, a fermentation liquor in thepipeline can also be concentrated online by adding a hollow fiber material or an embedded permeable membrane into the pipeline; therefore, physiological phenomena, such as constipation, caused in a disease state can be simulated. According to the reactor for culturing the intestinal flora and the method for culturing the intestinal flora by utilizing the reactor, the pipeline is arranged in a spiral manner, so that the space is compressed and the equipment space is saved. As parallel connection of multiple pipelines can be supported, and the multiple pipelines can be placed in the same jacket, parallel experimental temperatures are consistent, and the accuracy of experimental results is improved. According to the invention, researches can be carried out by correspondingly inoculating different floras into multiple intestinal segments and feeding different materials into the multiple intestinal segments.