The invention belongs to the technical field of gene engineering, and particularly relates to application of Arabidopsis thaliana NAC 103 gene to seed germination adjustment. NAC103 mutant plants nac103-d1 and nac103-e4 and NAC103 overexpression transgenic plant NAC103-OE1 and NAC103-OE3 are screened through a genetic engineering technological means; compared with wild-type Arabidopsis thaliana WT, the germination rates of the three kinds of gene plants are similar when no exogenous abscisic acid is added is discovered through biological statistics and analysis, but after the exogenous abscisic acid is added, the NAC103 mutant seed germination rate is high, while NAC103 overexpression transgenic plant seed germination is remarkably inhibited; the Arabidopsis thaliana NAC103 gene has the important biological function in the seed germination adjustment process under mediation of abscisic acid, the capability of the plant to resist adversity stress response is advantageously improved, and the seed selection of stress resistant good strains of crop is accelerated.