The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting flumetralin, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate, a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad. A flumetralin monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A flumetralin hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the quality control area. A flumetralin hapten is obtained in the modes that 2-chlorine-1,3-binitro-5-benzotrifluoride reacts with 3-amidogen-3-(2-chlorine-6-fluorophenyl)-propionic acid to generate 3-(2-chlorine-6-fluorophenyl)-3-(2,6-binitro-4-trifluoromethyl-anilino)-propionic acid, and then the 3-(2-chlorine-6-fluorophenyl)-3-(2,6-binitro-4-trifluoromethyl-anilino)-propionic acid reacts with iodoethane. The time resolution fluorescent immunochromatographic test strip and a detecting method have the advantages that operation is easy, sensitivity is high, the detecting speed is high, and the cost is low, and the flumetralin in large-batch samples can be detected quickly.