The invention relates to a cryoprotectant for red blood cells used immediately after being resuscitated and a using method, and relates to the technical field of cells. The cryoprotectant for the redblood cells comprises 0.25-0.3 mol/L of trehalose, 5-7% (v/v) of dimethylsulphoxide and 1-3% (v/v) of human serum albumin. The cryoprotectant for the red blood cells is simple in ingredient, when thecells are cryopreserved, the speed of mixing between the red blood cells and the cryoprotectant for the red blood cells does not need to be controlled strictly, and the red blood cells and the cryoprotectant are mixed directly, the cryopreserving method is simple and convenient, and consumed time is shorted. The red blood cells which are cryopreserved by the cryoprotectant can be used after beingresuscitated and are not required to be washed, troublesome and time-consumed washing steps in a glycerinum cryopreserving method are omitted, and demands of emergency blood users are met.