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发明公开
CN109136062A 数字流体样品分离设备和用于一步定量样品分析的方法 无效 - 撤回

数字流体样品分离设备和用于一步定量样品分析的方法
摘要：
本发明展示了一种用于分离、数字化和分析流体样品的数字分离(DS)芯片。所述DS芯片包括制备和区室化所述流体样品以进行分析的流体层。邻近孔的峭壁结构撇取所述流体样品，并且防止可干扰流体样品分析的颗粒进入所述孔。撇取后的流体样品分析在所述孔中进行，并且可收集终点数据，且将所述数据用于非常快地确定所述流体样品中所需组分的初始浓度。使用所述的设备和方法，可制备、数字化、区室化、测定流体样品，并在大约30分钟内收集所述终点数据。可易于改造所述设备和方法，以提供样品的平行处理。
摘要（英）：

A Digital Separation (DS) chip for separating, digitizing and analyzing a fluid sample is presented. The DS chip includes a fluidic layer that prepares and compartmentalizes the fluid sample for analysis. Cliff structures that are adjacent to wells skim the fluid sample and prevent particles, which may interfere with fluid sample analysis, from entering the wells. Skimmed fluid sample analysis occurs in the wells and endpoint data can be collected and used to determine an original concentration of a desired component in the fluid sample very quickly. Using the described apparatus and methods,a fluid sample can be prepared, digitized, compartmentalized, assayed and the endpoint data collected in about 30 minutes. The apparatus and methods can easily be adapted to provide parallel processing of a sample.

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