The invention relates to a shear model box with an adjustable natural vibration frequency, which comprises a layered box body and frequency regulating mechanisms arranged outside two opposite planes of the layered box body, wherein each frequency regulating mechanism comprises a side limiting steel plate (3) with a variable thickness and a limiting support (1) for fixing the side limiting steel plate (3), the side limiting steel plate (3) is fixed outside the layered box body through the limiting support (1), and shear stiffness is thus provided for the shear model box when relative horizontalsliding occurs between layers under vibration excitation. Compared with the prior art, the shear model box with the adjustable natural vibration frequency has the advantages that the frequency of themodel box can be regulated quickly and effectively; the test time is greatly shortened; the test accuracy is improved; the test cost is reduced and the like.