The invention provides a method, a system, a storage medium and a terminal for constructing an auxiliary pathological slice mold, comprising the following steps: constructing a 3D model of a tumor based on medical image data; constructing the main body model matched with 3D model; constructing a cover model matched with the main body model; constructing a border model matching the main body modeland the cover model; based on the body model, the lid model and the border model generating an auxiliary pathological slice mold. The auxiliary pathological slice mold construction method, the system,the storage medium and the terminal of the invention can construct the auxiliary case slice mold to realize one-to-one correspondence between the preoperative medical image and the postoperative pathological slice, thereby verifying the accuracy of the tumor boundary delineated on the preoperative medical image.