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发明公开
CN109207426A 一种将人造血祖细胞转变为造血干细胞的方法 有权

一种将人造血祖细胞转变为造血干细胞的方法
摘要：
本发明公开了一种利用人造血祖细胞制备人造血干细胞的方法，包括以下步骤：S1从人脐带血获取CD34+CD90-和CD34+CD45RA+脐带血造血祖细胞；S2所述CD34+CD90-和CD34+CD45RA+脐带血造血祖细胞悬浮接种于脐带血造血干细胞专用转化培养基中培养，所述脐带血造血干细胞专用转化培养基采用StemSpan SFEM II无血清培养基，添加100ng/ml SCF，100ng/ml FLT3，50ng/ml TPO；24孔板中细胞接种密度为：CD34+CD90-细胞为0.55x104/孔，CD34+CD45RA+细胞为0.13x104/孔；添加MS-275 1μM；置于37℃,5％CO2培养箱培养。本发明的有益效果：利用人造血祖细胞制备的造血干细胞数量高，且具备各谱系分化潜能，能够为临床应用提供造血干细胞供体。
摘要（英）：

The invention discloses a method for preparing human hematopoietic stem cells by using human hematopoietic progenitor cells, comprising the following steps: S1 preparing CD34+CD90- and CD34+CD45RA+ umbilical cord hematopoietic progenitor cells from human umbilical cord blood; S2) suspending the CD34+CD90- and CD34+CD45RA+ umbilical cord hematopoietic progenitor cells in a medium specially used forumbilical cord hematopoietic stem cells transformation and culturing the cells, the medium adopting a StemSpan SFEM II serum-free culture medium and 100ng/ml SCF, 100ng/ml FLT3 and 50ng/ml TPO beingadded into the medium, wherein the cell seeding density in 24-well plate is 0.55 *10<4> cells/well for CD34+CD90-cells, and 0.13 *10<4> cells/well CD34+CD45RA+ cells; 1[mu]M of MS-275 is added, and culture is performed at 37 DEG C in an incubator with the CO2 concnetration being 5%. The method has the beneficial effects that the hematopoietic stem cells prepared by using the human hematopoietic progenitor cells are high in quantity and have the differentiation potential of each lineage, and can provide a hematopoietic stem cell donor for clinical application.

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