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Invention Publication
CN109313196A 用于推断对象中的信号转导途径的转录因子的活性的方法 审中-实审

用于推断对象中的信号转导途径的转录因子的活性的方法
Abstract:
本发明涉及一种用于推断对象中的信号转导途径的转录因子活性的方法。所述方法包括执行(101)第一染色以检测对象样本的细胞中的转录因子，并且执行(103)第二染色以检测由样本中的细胞中的转录因子的靶基因编码的蛋白。所述方法还包括基于第一染色和第二染色来对样本中显示转录因子的核存在和靶基因编码的蛋白的存在的细胞进行量化(103)，并且基于所述量化来推断(104)所述对象中的转录因子的活性。这允许所提出的方法更可靠地推断对象中的转录因子的活性。
Abstract(English):

The present invention relates to a method for inferring activity of a transcription factor of a signal transduction pathway in a subject. The method comprises performing (101) a first staining for detecting the transcription factor in cells of a sample of the subject and performing (102) a second staining for detecting a protein encoded by a target gene of the transcription factor in cells of thesample. The method further comprises quantifying (103) cells of the sample that show both a nuclear presence of the transcription factor and a presence of the target gene-encoded protein based on thefirst staining and the second staining, and inferring (104) the activity of the transcription factor in the subject based on the quantifying. This allows the presented method to more reliably infer the activity of the transcription factor in the subject.

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