利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN109371139A 一种基于高通量测序技术用于检测甲状腺癌致病相关基因变异的引物及其应用 审中-实审

一种基于高通量测序技术用于检测甲状腺癌致病相关基因变异的引物及其应用
摘要：
本发明提供一种基于高通量测序技术用于检测甲状腺癌致病相关基因变异的试剂盒。本发明为针对甲状腺癌相关基因的变异检测，包括对BRAF、NRAS、KRAS、HRAS、TERT、TP53 6个基因的18个突变位点和CCDC6/RET、NCOA4/RET、PAX8/PPARg 3个融合基因进行检测。本发明利用两轮多重PCR来构建上机测序文库，针对以上九个基因的靶标区域设计特异性引物用于第一轮多重PCR，通用测序序列引物用于第二轮多重PCR。本发明还公开了包含上述引物序列，建库体系在内的检测甲状腺癌致病相关基因变异的试剂盒，可以辅助诊断意义不明确的甲状腺结节病变。
摘要（英）：

The invention provides a kit for detecting thyroid cancer pathopoiesia related gene variation on the basis of a high throughput sequencing technology. The kit is used for variation detection of thyroid cancer related genes, including 18 mutation sites of 6 genes comprising BRAF, NRAS, KRAS, HRAS, TERT and TP53 and 3 fusion genes comprising CCDC6/RET, NCOA4/RET and PAX8/PPARg. Online sequencing libraries are constructed by two round of multiple PCR, the specific primer is designed for target areas of the nine genes to be used in the first round of multiple PCR, and a general sequencing sequenceprimer is used in the second round of multiple PCR. The invention also discloses a kit, including primer sequences and library systems, for detecting thyroid cancer pathopoiesia related gene variation, and the kit can assist in diagnosing equivocal thyroid sarcoidosis lesions.

0/0