The invention relates to a method for detecting the content of bisphenol A by an electrochemical luminescent adaptor sensor. The method comprises the following steps of (a) providing an electrode, wherein DNA capturing single chains are fixed on the surface thereof; contacting the adaptor DNA of the bisphenol A with the electrode to form dsDNA, wherein the CDNA and the adaptor DNA realize partialcomplementary pairing; (b) performing co-incubation on an article to be detected containing bisphenol A with the electrode, so that the bisphenol A and the adaptor DNA are combined; (c) contacting theelectrode with two types of hair clip type DNAHP1 and HP2; forming long chain dsDNA through HCR reaction; marking the long chain dsDNA by an chemiluminescence probe; performing electrochemical luminescence detection, wherein the temperature for the heating step related in the detection process is provided through electrode heating. The method is simple and cheap; no mark exists; high sensitivityis realized; great potential is realized in field investigation aspects of different targets.