The invention discloses a method for inducing diapause of red-ear turtle eggs at low temperature, which comprises the following steps: 1) collecting red-ear turtle eggs: collecting turtle eggs, selecting turtle eggs with smooth egg shells, no depressions, no damages and no deformity, and placing the turtle eggs in an egg tray containing vermiculite; 2) cooling the turtle eggs, namely putting an egg tray containing the turtle eggs into an incubator, wherein the initial temperature in the incubator is the same as the temperature of the eggs; after putting the turtle eggs tray into the incubator,lowering gradually the temperature to 16.5-17.5 DEG C, and maintaining the temperature 3) nursing the turtle eggs, wherein the turtle eggs are inspected once every 3 to 5 days, the humidity of the vermiculite is adjusted, the humidity is kept stable, the mildewed or deteriorated eggs are removed in time, and the cleanness and the sanitation of an egg tray are kept. By utilizing the method, the diapause time of the red-ear turtle eggs can reach 15 days, the hatching rate can reach more than 50 percent, the cost is low, the operation is convenient, the environment is protected and safe, and powerful support is provided for scientific experiments using the red-ear turtle eggs as test materials.