The invention discloses a traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials of herba patriniae,dandelions and radix gentianae, and is used for resisting grouper iridovirus Guangxi strains. The traditional Chinese medicine composition is used as an effective action ingredient of medicines for preventing and treating the grouper iridovirus Guangxi strains. Through reasonable compatibility, the situation that the grouper iridovirus Guangxi strains are adsorbed to host cells can be interfered,the grouper iridovirus Guangxi strains can be prevented from penetrating cells, and the grouper iridovirus Guangxi strains can be restrained from duplication and synthesis in the host cells, so that the effect of resisting the grouper iridovirus Guangxi strains can be achieved; and the traditional Chinese medicine composition has the characteristics of being high in effective rate, safe and free from toxic and side effects.