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发明公开
CN109613093A 基于DNA纳米三棱柱构建组蛋白乙酰转移酶电化学发光生物传感器及其应用 有权 转让

  • 专利标题： 基于DNA纳米三棱柱构建组蛋白乙酰转移酶电化学发光生物传感器及其应用
  • 专利标题（英）： Manufactured histone acetyltransferase electrochemiluminescence biosensor based on DNA nano-prism and application of histone acetyltransferase electrochemiluminescence biosensor
  • 申请号： CN201910096484.7
    申请日： 2019-01-21
  • 公开(公告)号： CN109613093A
    公开(公告)日： 2019-04-12
  • 发明人: 胡宇芳 , 张青青 , 胡丹丹 , 马少华 , 郭智勇 , 王邃
  • 申请人： 宁波大学
  • 申请人地址： 浙江省宁波市江北区风华路818号宁波大学
  • 专利权人： 宁波大学
  • 当前专利权人： 山东博科诊断科技有限公司
  • 当前专利权人地址： 250100 山东省济南市中国（山东）自由贸易试验区济南片区港兴路9号博科集团总部2号楼4层A区402
  • 主分类号： G01N27/327
  • IPC分类号： G01N27/327 ;  G01N27/48
基于DNA纳米三棱柱构建组蛋白乙酰转移酶电化学发光生物传感器及其应用
摘要：
本发明公开了基于DNA纳米三棱柱构建组蛋白乙酰转移酶电化学发光生物传感器及其应用，具体步骤如下：(1）HAT-适配体反应：①乙酰化反应，分别取HATp300与多肽，乙酰辅酶A在磷酸缓冲溶液中(PBS，10mM，pH7.0)充分混合；②向①的反应溶液中加入CoA适配体；③向②的溶液中加入cDNA；(2)电化学发光传感器的制备：a.Au电极；b.prism/Au电极；c.cDNA/prism/Au电极；d.H1-H2/cDNA/prism/Au电极；e.Ru/H1-H2/cDNA/prism/Au电极。在乙酰化反应中，改变p300浓度及其小分子抑制剂浓度，探究所制备的一系列传感器对电化学发光信号的影响。优点是特异性好、灵敏度高、检测速度快、结果准确可靠、成本低。
摘要（英）：

The invention discloses a manufactured histone acetyltransferase electrochemiluminescence biosensor based on a DNA nano-prism and application of the histone acetyltransferase electrochemiluminescencebiosensor. The histone acetyltransferase electrochemiluminescence biosensor based on the DNA nano-prism is manufactured through the specific steps that (1) HAT-aptamer reaction is carried out, specifically, firstly, acetylation reaction is carried out, HATp300 and polypeptide are separately selected, and acetylcoenzyme A is fully mixed in a phosphate buffer solution (PBS, 10mM, pH7.0); secondly, CoA aptamer is added to the reaction solution in the first step; and thirdly, cDNA is added to the solution in the second step; and (2) the electrochemiluminescence sensor is manufacture through a. Auelectrode, b. prism/Au electrode, c. cDNA/prism/Au electrode, d. H1-H2/cDNA/prism/Au electrode and e. Ru/H1-H2/cDNA/prism/Au electrode. In the acetylation reaction, the concentration of p300 and the small molecule inhibitor concentration thereof are changed, and the effect of a series of sensors on an electrochemiluminescence signal is investigated. The manufactured histone acetyltransferase electrochemiluminescence biosensor based on the DNA nano-prism and the application of the histone acetyltransferase electrochemiluminescence biosensor have the advantages of good specificity, high sensitivity, fast detection speed, accurate and reliable results and low cost.

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