The invention discloses a splicing structure of a single panel and a manufacturing method thereof. The splicing structure of the single panel comprises a first product unit and a second product unit.The first product unit and the second product unit are respectively arranged in two or more columns and two or more rows of mirror direction staggered layout in the X-axis direction and the Y-axis direction. The invention separates each product unit of the single panel into the first product unit and the second product unit, the circuit graphic layer of the first product unit is upward, the circuit graphic layer of the second product unit is downward. The circuit graphic layers of the first product unit and the second product unit are arranged in two or more columns and two or more rows of mirror direction staggered layout in the X-axis and Y-axis directions, respectively, and corresponding solder layer and/or strengthening layer dose corresponding layout design. The invention can effectively improve the problem that the product is easy to wrinkle and fold due to the thin overall thickness of the original single panel splicing structure and the plate surface is curled up and deformed after the whole top solder protection layer is manufactured, thereby improving the production yield of the single panel.