The invention relates to a composition containing probiotic bacteria and prebiotics and an application thereof. The composition comprises, by weight, 10-50 parts of lactose, 10-40 parts of maltodextrin, 5-20 parts of fructo-oligosaccharide, 5-20 parts of isomaltooligosaccharide, 0.5-5 parts of lactobacillus plantarum, 0.5-5 parts of bifidobacterium lactis, 0.5-5 parts of lactobacillus acidophilusand 0.5-5 parts of lactobacillus rhamnosus. According to the invention, specific prebiotic bacteria and probiotics are selected to be combined according to a specific ratio, so that the probiotic bacteria and the prebiotics can generate synergistic effect, and intestinal flora can be obviously improved after being taken by people with poor intestinal tract, so that the intestinal tract function can be further improved.