The invention discloses a method for improving a seedling transplantation survival rate and relates to the technical field of urban landscaping. The problems that the survival rate is low due to rootrot and water shortage in the conventional seedling transplantation process and the like are solved. According to key points of the technical scheme, the method comprises the following steps: a, primary root breaking; b, bacterial colony culture; c, repeated root breaking; d, pruning; e, soil ball digging; f, soil ball packing; g, pit digging; h, soil ball burying; and i, watering. By virtue of repeated root breaking and subsequent culture, fibrous roots in soil balls are developed, beneficial bacterial colony is cultured on the fibrous roots, and the growth environment of the root system is improved, so that the phenomena such as root rot and water shortage difficultly occur after transplantation, and the survival rate of the nursery stock is improved.