The invention discloses an antibacterial and wear-resistant plastic flooring and a preparation method thereof. The preparation method comprises the steps of attaching an anion to a plant fiber line; then weaving a plant silver fiber line to obtain a surface mesh layer; attaching the surface mesh layer to an obtained base layer; and smearing a surface liquid mixed with nano silicon dioxide and nanotitanium dioxide on the base layer to obtain the antibacterial and wear-resistant plastic flooring. The plastic flooring is more resistant to bacteria through the bactericidal effect of the anion; and silicon dioxide and titanium dioxide are disposed in the surface layer of the plastic flooring, so that the surface layer is more wear-resistant.