The invention discloses a non-invasive pressure-applying human body pulse information collecting device, in particular to a pulse wave collecting device based on a stepping motor, a pressure sensor and a flexible coupling pipeline. By integrating the high-precision stepping motor, the high-performance pressure sensor, the flexible pipeline with excellent coupling property with human skin and a control and data processing circuit, the device solves the problems that a prior pulse wave collecting device has low collecting precision and cannot be tightly attached to an arm. The device mainly comprises a shell with a sleeve, wherein a support plate is arranged in the shell; the stepping motor is fixed on the support plate; the bottom of the stepping motor is contacted with the surface of the flexible pipeline; a seam is formed in the bottom of the shell; the flexible pipeline is clamped in the seam; physiological saline is filled in the flexible pipeline; a pressure sensor probe is sealedin the flexible pipeline; an collecting and storing control circuit is fixed in the shell; and the collecting and storing control circuit comprises a collecting module, an amplification module, a maincontrol module and a storing module. Collected signals are connected to an upper computer through a USB for real-time waveform display and storage.