The invention relates to the field of substance separation detection and provides a synchronous detection method of coniferyl alcohol and pinoresinol. Absorbance values of coniferyl alcohol and pinoresinol in a to-be-detected sample are measured by using high performance liquid chromatography, wherein the detection wavelength is 280 nm and the high-performance liquid chromatography employs gradient elution and the eluent in the gradient elution includes methanol and water; according to a standard curve of coniferyl alcohol and the absorbance of the coniferyl alcohol in the coniferyl alcohol, the concentration of the coniferyl alcohol in the to-be-detected sample is obtained; and on the basis of the standard curve of pinoresinol and the absorbance of pinoresinol in the to-be-detected sample, the concentration of the pinoresinol in the to-be-detected sample is obtained. Therefore, the coniferyl alcohol and pinoresinol in the to-be-detected sample can be separated effectively; and the corresponding concentrations of the coniferyl alcohol and pinoresinol can be detected respectively.